Wake Tech offers a variety of non-degree, short classes and certificate training programs in the biotechnology field, including hands-on training in biomanufacturing and biopharmaceutical operations in a simulated industrial, current good manufacturing practices (cGMP) environment.
Course Details & Registration Information
- Gain an understanding of the biological principles behind microbial fermentation, growth, and genetic modification.
- Summarize and apply Current Good Manufacturing Practice (CGMP) considerations in fermentation.
- Learn how to optimize growth medium for microbial growth and describe the nutritional requirements for media preparation.
- Describe the basics of fermentor design, instrumentation, and control.
- Identify the components of a small scale fermentor system and gain hands-on experience with operating the system to run a fermentation cycle.
- Demonstrate taking analyte measurements to monitor the growth of cultures.
- Day 1 Lectures
a. Biomanufacturing process overview
b. A brief overview of cell culture
c. Introduction to fermentation processes (history)
d. Current Good Manufacturing Practices (CGMP) related to fermentation processes
e. Industrial microorganisms and their products
f. Phases of cell growth
g. Recombinant technology
- Day 1 Labs
a. Review lab safety
b. Refresh a culture
c. Prepare shaker flask media
d. Identify nutrition components of various media
e. Arabinose usage as an inducer
- Day 2 Lectures
a. Factors that affect cell growth
b. Fermentor design requirements
c. Parts of fermentor
d. Nutritional requirements for aerobic fermentation
e. Sterilization principles
f. Fermentor design
- Day 2 Labs
a. Inoculation and monitoring shaker flasks for growth
b. Sterilization investigation
c. Bioreactor component identification
- Day 3 Lectures
a. Fermentation operation modes
b. Fermentation instrumentation and control
c. Fermentation support systems
d. Single-use technologies
- Day 3 Labs
a. Inoculation and monitoring the bioreactor
b. Results of the sterilization experiment
c. OD600 analysis, Glucose analysis
d. Percent solid/biomass analysis
- Attendance 90% or above
- Active participation in all labs, class discussions, and presentations.
- Bioprocess Technician
- Bioprocess Engineer
- Manufacturing Associate
- Fermentation Technician
- Fermentation Lab Analyst
- Elements of Current Good Manufacturing Practices in Biomanufacturing Processes - SEF-79524